Reliable Propagation of Persian Walnut Varieties Using SSR Marker-based True-to-type Validation

Author:

Nickravesh Masoomeh Hosseini1,Vahdati Kourosh1,Amini Fatemeh2,Di Pierro Erica A.3,Amiri Reza2,Woeste Keith4,Arab Mohammad M.1

Affiliation:

1. Department of Horticulture, College of Aburaihan, University of Tehran, Tehran, Iran

2. Department of Agronomy and Plant Breeding, College of Aburaihan, University of Tehran, Tehran, Iran

3. Research and Innovation Centre, San Michele all’ Adige, Edmund Mach Foundation, Trento, Italy

4. USDA Forest Service, Hardwood Tree Improvement and Regeneration Center, Department of Forestry and Natural Resources, Pfendler Hall, Purdue University, 715 West State Street, West Lafayette, IN 47907-2061

Abstract

Simple sequence repeat (SSR) markers were used to authenticate ramets of 11 Persian walnut (Juglans regia L.) varieties. All varieties and 28 of their ramets (n = 39) were genotyped with 17 SSR markers. The genetic profiles revealed two off-types: the ramets Serr 4 (S4) and Vina 1 (V1). SSR fingerprints individuating 11 walnut varieties were possible using 13 polymorphic SSRs that could be used in the future to identify clones of these varieties. Except for ‘Chandler’, each cultivar could be distinguished using a combination of two SSR loci. This result emphasizes the efficacy of the SSR markers in true-to-type validation of walnut orchards.

Publisher

American Society for Horticultural Science

Subject

Horticulture

Reference13 articles.

1. Walnut: Past and future of genetic improvement;Bernard,,2018a

2. Analysis of genetic diversity and structure in a worldwide walnut (Juglans regia L.) germplasm using SSR markers;Bernard,,2018b

3. Characterization of 14 microsatellite markers for genetic analysis and cultivar identification of walnut;Dangl,,2005

4. Isolation of plant DNA from fresh tissue;Doyle,,1990

5. Analysis of genetic diversity among some Persian walnut genotypes (Juglans regia L.) using morphological traits and SSRs markers;Ebrahimi,,2011

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