Ploidy Levels and DNA Contents of Bougainvillea Accessions Determined by Flow Cytometry Analysis

Author:

Li Haiyan1,Niu Junhai1,Sun Luping2,Li Ya2,Leng Qingyun2,Chen Jinhua2,Zhang Jinran3,Yanan Yanan3,Ma Chao3,López Hernán Ariel4

Affiliation:

1. Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571737, China; and The Engineering Technology Research Center of Tropical Ornamental Plant Germplasm Innovation and Utilization, Hainan Province, Danzhou, 571737, China

2. Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571737, China

3. College of Horticulture, China Agriculture University, Beijing, 100191, China

4. Multidisciplinary Workshop on Vascular Plants, Border Ecology Laboratory, University of Flores, Sede Comahue (UFLO), Rio Negro, Argentina

Abstract

Bougainvillea Comm. ex Juss. (Nyctaginaceae; Bougainvillea) is a popular ornamental plant with vigorous growth, luxuriant blooming, colorful bracts, and a high tolerance to the stresses of temperature, drought, and soil pollution, and thus is widely cultivated in tropical and subtropical regions. However, the paucity of information on ploidy and the genomic constitution is a significant challenge to genome research and cultivar improvement. We present a flow cytometry method for ploidy detection in bougainvillea based on evaluating different lysates and tissues, identify the ploidy level of a batch of bougainvillea accessions, and infer the genome size of horticultural species Bougainvillea glabra, Bougainvillea spectobilis, and Bougainvillea peruviana. The results show that tender leaves and woody plant buffer (WPB) were optimal for flow cytometry analysis. The 2C nuclear DNA amounts in 176 bougainvillea accessions ranged from 4.66 ± 0.04 to 12.26 ± 0.1 pg, which represents 161 diploids, 13 triploids, 1 tetraploid, and 1 di-tetraploid mixoploid. For B. glabra, B. spectobilis, and B. peruviana, the mean 1C values were 3.201, 3.066, and 2.915 pg, respectively. The genome size of B. glabra was significantly larger than that of B. peruviana (P = 0.0004), but had no significant variation with that of B. spectobilis (P = 0.1061). These results reveal fundamental cytogenetic information for bougainvillea that are beneficial to whole-genome sequencing and hybrid breeding programs.

Publisher

American Society for Horticultural Science

Subject

Horticulture

Reference17 articles.

1. Bougainvillea genus: A review on phytochemistry, pharmacology, and toxicology;Abarca-Vargas,,2018

2. Polyploidy and genome evolution in plants;Adams,,2005

3. Phylogeny and taxonomic synopsis of the genus Bougainvillea (Nyctaginaceae);Bautista,,2022

4. Comparative analysis of complete chloroplast genome sequences of wild and cultivated Bougainvillea (Nyctaginaceae);Bautista,,2020

5. Flow cytometry as tool in plant sciences, with emphasis on genome size and ploidy level assessment;Bourge,,2018

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3