Cross Breeding New Cultivars of Early-flowering Multiflora Chrysanthemum Based on Mathematical Analysis

Abstract

Conventional crossbreeding remains an effective technique for chrysanthemum (Chrysanthemum ×morifolium Ramat.) breeding. However, there are always many problems when breeding chrysanthemum because of its complex genetic background, such as difficulty matching parents, selecting superior hybrid progenies, quantitatively describing certain target traits, and evaluating breeding results. A recent mathematical analysis method is an effective method for evaluating plant breeding progress. In this study, we used 505 multiflora chrysanthemum germplasm resources as test materials; we divided the flowering time into five groups using a grading analysis method, including extremely early group (genotypes that flowered when daylength was longer than 13.5 hours), early group (genotypes that flowered when daylength was 13.5–12.0 hours), medium group (genotypes that flowered when daylength was 12.0–11.0 hours), late group (genotypes that flowered when daylength was 11.0–10.0 hours), and extremely late group (genotypes that flowered when daylength was shorter than 10.0 hours). Moreover, the breeding objective was to breed early-flowering genotypes. Using 15 phenotypic characters as evaluation factors, 37 excellent genotypes, including four early-flowering genotypes, were screened out from the aforementioned resources according to an analytic hierarchy process (AHP) and weighting of the gray relational grade. We selected one early-flowering genotype and eight medium-flowering genotypes from these 37 genotypes and matched six hybridized combinations based on the genetic distance between genotypes calculated by the Q cluster analysis method. We used a comprehensive evaluation method combining AHP and the gray relational analysis (GRA) method for the evaluation of 367 progenies. Moreover, we screened out 52 superior hybrids, including 36 early-flowering hybrids. The results of this study demonstrate that the mathematical analysis method is an immensely effective method to breed new cultivars of early-flowering multiflora chrysanthemum. This study also provides an effective method to define and improve the flowering time of other cultivated plants.