Induced Polyploidy in Diploid Ornamental Ginger (Hedychium muluense R. M. Smith) Using Colchicine and Oryzalin

Abstract

The ploidy level of H. muluense, a diploid (2n = 2x = 34) and dwarf ornamental ginger species, has been determined and is reported for the first time. Oryzalin and colchicine were successfully used to induce polyploidy in Hedychium muluense in vitro. Embryogenic cell lines were treated with oryzalin (30, 60, or 120 μM) and colchicine (2.5, 5, or 10 mm) for 24, 48, or 72 h. The control contained no antimitotic agent. Flow cytometry, chloroplast count, and stomatal frequency were more effective and reliable than stomatal length as methods for assessing ploidy. Overall, oryzalin was more effective than colchicine in inducing polyploidy. The highest induction frequency (15%) of tetraploidy was achieved when embryogenic callus was exposed to 60 μM oryzalin for 72 h. For colchicine, exposure of embryogenic callus to the 2.5 mm colchicine for 24 h was the most effective in creating tetraploid (13%) plants.