Thidiazuron- and Zeatin-induced High-frequency Shoot Regeneration from Leaf and Shoot-tip Explants of Strawberry

Author:

Haddadi Fatemeh1,Aziz Maheran Abd12,Kamaladini Hossein13,Ravanfar Seyed Ali1

Affiliation:

1. 1Department of Agriculture Technology, Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Selangor, 43400, Malaysia

2. 2Laboratory of Plantation Crops, Institute of Tropical Agriculture, Universiti Putra Malaysia, Serdang, Selangor, 43400, Malaysia

3. 3Department of Biology, Faculty of Science, University of Zabol, Zabol, Sistan and Bloochestan, Iran

Abstract

Strawberry (Fragaria ×ananassa cv. Camarosa) was evaluated to determine a high-frequency shoot regeneration response for leaf and shoot-tip explants. For direct organogenesis from strawberry leaves, combinations of moderate concentrations of thidiazuron [TDZ (0, 2, and 4 μm)] and 6-benzylaminopurine [BAP (0, 4, and 9 μm)] added into medium containing Murashige and Skoog (MS) basal salts were compared. The most shoots regenerated per leaf explant were observed with 4-μm TDZ. Regeneration from shoot tips was evaluated with 0-, 2-, 4-, 8-, and 16-μm zeatin, kinetin, or 6-α,α-dimethylallylamino purine (2ip) tested individually. Optimum shoot proliferation was achieved from shoot-tip explants on medium containing 4-μm zeatin. Rooting was best without cytokinins in the medium; however, adequate rooting was obtained on the 4-μm zeatin treatment as well.

Publisher

American Society for Horticultural Science

Subject

Horticulture

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