Effects of Plant Growth-promoting Microorganisms on the Early Growth of Kentucky Bluegrass under Drought and Salinity

Abstract

Drought and salinity affect turfgrass growth and development adversely. Plant growth-promoting microorganisms (PGPMs) have been shown to have the capability of improving resistance to biotic stressors in plants. The objective of this research was to determine the efficacy of six commercial PGPMs on enhancing the drought and salinity resistance of kentucky bluegrass (Poa pratensis). The six PGPMs evaluated were Beauveria bassiana (strain GHA), Bacillus subtilis (strain GB03), Azadirachtin, Bacillus firmus (strain 1-582), Trichoderma harzianum Rifai (strain T-22) combined with Trichoderma virens (strain G-41), and Bacillus subtilis (strain QST713). Three cultivars—Kenblue, Moonlight, and Waterworks—were seeded in the greenhouse. Two-week seedlings were exposed to saline (sodium chloride at 6 dS⋅m–1 three times per week) or drought (tap water once per week) conditions, and no stress (irrigated with tap water three times per week) for 4 weeks. Results show that drought and salinity inhibited turf growth, with the greatest reduction in root dry weight (50.3% in drought conditions and 31.4% in saline conditions). ‘Kenblue’ performed better than ‘Waterworks’ and ‘Moonlight’ in all growth indices except for root length. Beauveria bassiana and B. subtilis had a similar or better result in enhancing turfgrass growth and development compared with the untreated turf under stress. Our results suggest that certain PGPMs have the potential to improve abiotic stress resistance in turfgrass.