Genotype TNF-α(-308) and Silicosis on Factory Workers in Vietnam in 2020

Abstract

The studFigy aims to determine the TNF-α single-nucleotide polymorphism TNF-α (-308) andassess the association of TNF-a(-308) SNP with the risk of silicosis among workers directly exposed tosilica dust in Vietnam. A study was undertaken among 78 cases with silicosis and 103 controls withoutsilicosis in Vietnam. Blood samples were collected for genomic DNA extraction from each subject. Thephenotyping of TNF-α(-308) was performed using polymerase chain reaction‐based restriction fragmentlength polymorphism (PCR‐RFLP) and dye termination sequencing. Results: The average exposure timeof the case group was slightly higher than that of the control group (12.46 ± 6.732 years vs. 12.09 ± 7.854years). The majority of genotypes in both silicosis and non-silicosis was GG. When analyzing theconcentration of TNF-α in the study participants' blood, it is shown that the average concentration of TNF-α in the case group was higher than that in the control group. The genotype AG in the case group was1.368 times higher than that in the control group. The percentage of all A alleles in the case group withsilicosis was 1.342 times higher than the control group without the disease, similar to previous studies.Conclusion: The majority of genotypes in both groups was GG. The average concentration of TNF-α inblood, genotype AG, and the percentage of all A alleles in the case group was higher than that in thecontrol group.