Priming edilmiş endüstriyel kenevir (Cannabis sativa L.) tohumlarının in vitro çimlenmesinin optimize edilmesi

Abstract

Germination under in vitro conditions for industrial hemp (Cannabis sativa L., Cannabaceae) is highly significant for the application of biotechnological tools like genome editing. Therefore, seeds were surface sterilized followed by priming with hydrogen peroxide (H2O2) and sterile distilled water (dH2O) for 24, 48, and 72 h. The primed seeds were inoculated on the Murashige and Skoog (MS) medium enriched with 1.0 mg/L benzyl amino purine (BAP) and 200 mg/L antibiotics. Exposing seeds to H2O2 was superior and 100% germination was observed. Whereas hydropriming resulted in 30-52.5% germination with a maximum of 48h priming time. The results on shoot counts revealed a maximum of 1.98 shoots from the combination of 3.0% H2O2 and 72h priming time. The results were also analyzed by constructing different statistical plots like box plots, normal plots, contour plots, and surface plots. The normal plots exhibited the significance of H2O2 concentration on both output variables. Whereas contour and surface plots classified the output data into different sub-groups and confirmed the results.