AKRİLAMİDE MARUZ KALAN SIÇANLARIN KALP DOKUSU ÜZERİNDE SELENYUMUN KORUYUCU ROLÜ

Abstract

Objective Acrylamide (ACR) is a harmful organic reactive compound widely used in different industrial fields. Sele- nium (SEL) is a trace element that takes part in some chemical and enzymatic reactions in both animals and human organisms and can cause some disorders in many systems such as the cardiovascular system in case of deficiency. The aim of this study is to investigate whether SEL has a protective effect on cardiac tissue in experimentally induced ACR toxicity in rats. Material and Method A total of 28 rats were randomly and equally divided into four groups: Control, SEL, ACR, SEL + ACR. At the end of the study, cardiac markers such as creatine kinase MB (CKMB), lactate dehydrogenase (LDH) and ischemia-modified albumin (IMA) from blood samples were measured. Oxidative stress markers such as total oxidant status (TOS), total antioxidant status (TAS) and oxidative stress index (OSI) in heart tissue were analyzed. In addition, inducible nitric oxide synthase iNOS activities were determined in heart sections using immunohistochemical methods. Results The ACR + SEL group showed a significant decrease in TOS, OSI, CKMB, LDH, IMA levels and significant increase in TAS level compared to the ACR group. In histopathological and immunohistochemical evaluation of the heart sections; Disorganization in cardiac muscle fibers, increased connective tissue between muscle fibers and irregular eosinophilia in muscle fibers were detected in ACR group. A decrease in histopathological findings was observed in the SEL treated groups compared to the ACR group. iNOS immunoreactivities; moderate marking was observed in the ACR group sections, while poor marking was observed in the ACR + SEL group. Conclusion We determined that ACR caused damage to the heart tissue of rats through various metabolic pathways and that these damages were reversed in the groups given the SEL.