SİNİR BÜYÜME FAKTÖRÜ İLE FARKLILAŞTIRILMIŞ PC12 HÜCRELERİNİN MORFOMETRİK VE FLORESANS ANALİZİ

Abstract

Objective PC12 is a rat pheochromocytoma cell line. These cells characteristically undergo differentiation when cultured with nerve growth factor (NGF). Depending on the dose of NGF, the length of neurite extensions changes. Thanks to this differentiation property, the cells are used in neuroscience and in modeling pathophysiological diseases such as Alzheimer's, Parkinson's, and Amyotrophic Lateral Sclerosis. However, literature studies showing the effect of NGF on neurite extensions formed in PC12 cells are very limited. This study aimed to investigate the effect of NGF on neurite extensions and cell viability depending on dose and incubation time. Materials and Methods In this study, PC12 cells were incubated with 50 ng/ml and 100 ng/ml NGF for 3, 6 and 7 days. The lengths of neurite outgrowths and dead cell ratios were calculated in incubated cells. Results The results showed that the length of neurite extensions and dead cell ratio increased depending on NGF doses and incubation time. When NGF incubation times were compared, no difference was found between 50 ng/ml NGF 6 days and 100 ng/ml NGF 3 days groups. Conclusion When the dead cell ratios and sizes of neurite extensions in the experimental groups are evaluated, it is thought that 100 ng/ml NGF and 3 days incubation time parameters are ideal for PC12 cell differentiation.