Effect of Adinopectin to MCF-7 Proliferation and Ghrelin Level

Abstract

Objective: Local and systemic factors have been shown to drive the growth of breast cancer cells in postmenopausal obese women who are at increased risk of estrogen receptor positive breast cancer. In obese postmenopausal women, it has been shown that circulating ghrelin hormone is decreased, produced by some tumor cells, and also affects tumor growth. In this study, we aimed to investigate the role of adiponectin administration in estrogen receptor positive MCF-7 breast cancer cell line and its effects on ghrelin levels. Material and Methods: The MCF-7 breast cancer cell line was propagated in 2D culture. The inhibition dose was determined by treating MCF-7 breast cancer cells with increasing concentrations of adiponectin (50-5000 ng/mL). Ghrelin levels were measured at 24th, 48th and 72nd hours by enzyme-linked immunosorbent assay (ELISA) by administering adiponectin at a concentration of 50, 100, 250 and 500 ng/mL to the MCF-7 breast cancer cell line. Results: In MCF-7 breast cancer cell line culture samples taken at 24th, 48th and 72nd hours, proliferation was found to decrease depending on 50, 100, 250 and 500 ng/mL adiponectin concentration. It was found that the amount of ghrelin increased depending on 50, 100, 250 and 500 ng/mL adiponectin concentration in MCF-7 breast cancer cell line culture samples taken at 24th, 48th and 72nd hours. Conclusion: In our study, we revealed the role of adiponectin, which has proven anti-carcinogenic properties in the MCF-7 breast cancer cell line (ER/PR positive) and also negatively correlated with obesity, and ghrelin, in ER positive breast cancers.