Changes in Plasma Amino Acid Levels in Crimean-Congo Hemorrhagic Fever Patients

Abstract

Objective: Crimean Congo Hemorrhagic Fever (CCHF) has an important place in viral hemorrhagic fever. Plasma amino acid (AA) levels of patients who were diagnosed with CCHF in the acute and convalescent period of the disease were investigated in this study. Methods: 35 patients were included in the study specific polymerase chain reaction (PCR) and/or IgM antibody positivity for CCHF virus. AA levels were measured in the plasma derived from the blood samples of the patient and control groups, using liquid chromatography-mass spectrometry (LC-MS/MS) technique. Results: In our study, we observed that plasma aspartate, glutamate, histidine, leucine, phenylalanine, tyrosine levels increased statistically significantly (p<0.05), while some AA levels decreased (p<0.05) in acute stage patients compared to the control groups. In addition, while there was an increase in plasma glutamate levels of convalescent patients compared to the control groups (p<0.05), there was a significant decrease in other AA levels (p<0.05).             Conclusion: Further studies to investigate the relationship between increased or decreased AAs in the plasma levels of these patients and the immune system are likely to contribute to a better understanding of the pathogenesis of the disease and to guide the approach to whether AA supplementation is necessary for treatment.                 Keywords: biochemical analysis, Crimean‐Congo hemorrhagic fever virus, metabolic analysis, research andanalysis methods