Antioxidative and Anti-inflammatory Effects of Daehwangmokdanphee-tang

Abstract

This study was conducted to investigate antioxidant and anti-inflammatory effects of Daehwangmokdanphee-tang (DHMDPT). The antioxidant activity was evaluated the total polyphenol contents and total flavonoid contents. Additionally we measured the ABTS radical scavenging activity, superoxide dismutase (SOD)-like activity and electron-donating ability with DHMDPT. In addition, inhibition of NO production in RAW 264.7 cells induced by LPS was evaluated to investigate the anti-inflammatory effect. The results of total polyphenol contents, total flavonoid contents was 52.87±12.52 mg/g and 7.20±4.49 mg/g in DHMDPT. Antioxidant activity results of DHMDPT through ABTS radical scavenging activity, superoxide dismutase (SOD)-like activity, and electron-donating ability are highly active in a dose-dependent showed high activity. The cell toxicity of DHMDPT was evaluated using the RAW 264.7 cells. LPS-induced inhibition of NO secretion was increased in DHMDPT in a dose-dependent manner. This result indicates that DHMDPT might have antioxidant and anti-inflammatory effects in vitro assay, and can be developed as a cosmetic material in the future.