Effect of supplementation of lutein and DL-methionine on certain haemato-biochemical parameters and serum concentration of corticosteronein captive Golden pheasants (Chrysolophus pictus)

Abstract

Twenty four male golden pheasants were divided into 4 groups of 6 each. First group basal diet contained no supplementary lutein or DL-methionine (M0C0). The diet of the birds in group M0C1 were supplemented with lutein (40 mg/kg DM) without supplementary DL methionine, whereas those in group M1C0 were supplemented only with DL-methionine (1.5g/ kg DM). Birds in group M1C1 were fed both the supplements. A feeding trial of 60 days duration was conducted. Heterophill counts was significantly lower (Pless than0.001) in groups M0C1 and M1C1 as compared to group M0C0 and M1C0 and lymphocyte count was higher (Pless than0.05) in lutein supplemented group as compared to non-lutein supplemented groups. The ratio of H: L was significantly higher (P less than 0.001) in lutein supplemented groups. Other haematological and serum biochemical parameters were not significantly different among the groups. Serum concentrations of corticosterone were lower (Pless than0.001) in groups M0C1 and M1C1 as compared to groups M0C0 and M1C0. It was concluded that supplementation of lutein (40mg/kg DM) but not DL- methionine (0.15% DM) improved the capability of the Golden pheasant to combat stress.