Histoenzymic distribution of phosphatases, dehydrogenases and esterasesin ileal peyer’s patches of neonatal buffalo calves

Abstract

The histoenzymic study was conducted on ileum of neonatal buffalo calves (n=15) and their age was estimated by observing their temporary and permanent dentition. Histoenzymic distribution of different enzymes was studied on ileal peyer’s patches of neonatal buffalo calves. Strong activity of AKPase was observed at the dome and capsule of the peyer’s patch lymphoid follicles at this age. However, moderate Lactic dehydrogenase (LDH) and SDH activity was observed within the ileal lymphoid follicles. A strong activity of Glucose-6-phosphate dehydrogenase enzyme was observed in the follicles at their periphery and within center of few follicles. Also a strong positive activity of diaphorases was observed in peripheral region of the follicle as fine granular reaction. The activity of enzymes has been discussed with the physiological function of the tissue..