Molecular detection of porcine circo virus type 2 and porcine parvo virus inpigs having reproductive problems and histopathologicalstudies in the tissue of aborted pig foetuses

Abstract

The present study was undertaken to detect the presence of porcine circovirus type 2 (PCV2) and porcine parvo virus (PPV) in 54 porcine aborted foetuses, stillborns and mummified foetuses. Foetal tissues of heart, lung, spleen and lymphnodes were used for extraction of total DNA and the presence of viral pathogens were confirmed through polymerase chain reaction (PCR) technique. Out of 54 samples, nine (16.6%) samples were positive for PCV2, eight (14.8%) samples were positive for PPV while co-infection with PCV2 and PPV was detected in four (7.4%) samples. The histopathological changes in the foetuses were found to be mostly necrosis in the cells of the developing organs viz. lungs, kidney, liver, heart, brain, spleen and lymphnodes. Lymphoid depletion was observed in the lymphoid follicles of the lymphnodes. Meningoencephalitis in the cerebrum was also seen in both PPV and PCV2 infected foetuses. The results indicated that PPV and PCV2 could be an important infectious agent in cases of porcine stillbirths, abortions and mummified foetuses.