Wistar Rat Pneumonia Model: Bacterial Dose, Incubation Time, Inoculation Technique and Laboratory Examination Parameters

Abstract

Background: Making animal models is important before research involving human diseases. This study aimed to prepare animal models of pneumonia through the intranasal technique with a focus on the effect of the dose of Klebsiella pneumoniae (Kp) and incubation period on diagnosing pneumonia. We use male Wistar rats 6-8 weeks, 200-300 grams as much as 64 that appropriate the inclusion and exclusion criteria. Methods: The research method was the pre-posttest group design to prove the creation of a Wistar Rat pneumonia model based on the dose and incubation time for diagnosing Pneumonia. There were On days 1 to 5, each of the 4 rats underwent a thoracic radiography (CXR) 64 rats model divided into 4 groups, N: 4 healthy; T1, T2 and T3 groups of rats were inoculated with 25, 50 and 100 µL of 0.5 McFarland intranasal Kp solution. A group of rats was then sacrificed, followed by retrobulbar blood collection (leukocyte-neutrophil count) and bacterial count/NaCL and lung tissue was taken histopathology and bacterial count/Brain Heart Infusion (BHI). The independent variables were the dose of Klebsiella pneumoniae (Kp) and the incubation period. The statistical analysis of the correlation of dose of bacteria, incubation time with leucocyte and neutrophils used One Way Anova, Kruskal Wallis if the data were abnormally distributed. The correlation is the dose of Kp, incubation period with chest radiography, histopathology, blood bacterial count (BBC) and pulmonary bacterial count (PBC) using the Chi-square test. Result: The results showed that the dose of Kp was positively and significantly correlated with the neutrophil count (p=0.030) and the histopathology (p=0.000). Similarly, the incubation period was positively and significantly correlated with chest radiography (p=0.000) and histopathology (p=0.013).