Determining Serum Amyloid-A (SAA), Haptoglobin (Hp), Tumor Necrosis Factor (TNF-α) and Interleukins-1 (IL-1β) and 6 (IL-6) Levels in Neonatal Calves with Amoebiasis

Abstract

Background: Amoebiasis is a pathogen organism which is well-known both in human and veterinary medicine. The aim of this study was to determine serum amyloid-A (SAA), haptoglobin (Hp), tumor necrosis factor (TNF-α) and interleukins-1 (IL-1β) and 6 (IL-6) levels in neonatal calves which were naturally infected with amoebiasis. Methods: In the study, 40 one to four-week-old calves with amoebiasis which were clinically diagnosed with diarrhea and were non-responsive to the treatment, and 10 healthy calves were used. From the stool samples taken from the calves which were clinically diagnosed with diarrhea, Entamoeba histolytica was detected by using ELISA kit. Serum was obtained from the blood taken from vena jugularis from all calves. Concentrations of SAA, Hp, TNF-α, IL-1β and IL-6 in serum were measured in ELISA by using commercial kits. Also, some enzymes measurements were made from serum. Result: Concentrations of SAA, Hp, TNF-α, IL-1β and IL-6 in calves with amoebiasis were higher (p less than 0.05) in comparison to the control group. To conclude, amoebiasis led to an increase in the concentrations of SAA, Hp, TNF-α, IL-1β and IL-6 in neonatal calves. We suggest that this might have stemmed from the inflammatory response against amoebiasis.