Estimation of Hormonal Seed Treatments on Enzyme Activities after Accelerated Ageing (Artificial Ageing) in Chickpea (Cicer arietinum L.)

Abstract

Background: Accelerated ageing, which is known to diminish seed viability and vigour in different seed crops, has been used to predict seed storability. The goal of this study was to see how accelerated ageing affected enzymatic characters of chickpea seeds (Variety Pusa 362). Seeds were exposed to accelerated ageing conditions by being heated to 35oC and 40oC and kept at 90% and 100% relative humidity at different duration i.e., 2, 4 and 8 days. After accelerating ageing pre sowing seed treatment with salicylic acid and gibberellic acid has done. The findings revealed that faster ageing causes the chickpea seed to degenerate, which is linked to a decline in enzymatic characters but hormonal seed treatments enhances enzymatic (amylase, catalase, peroxidase, dehydrogenase and protease) performance after ageing. Our result showed that antioxidant activity of aged seeds increased after seed treatment with plant growth hormones. Methods: The present experiment was conducted at state seed testing laboratory, Department of Genetics and Plant Breeding, SHUATS, Prayagraj, Uttar Pradesh during the year 2018 to 2020. In the experiment treatment were comprises with 37 treatment combination. The seeds are exposed to high temperatures and relative humidity in a chamber to speed up the ageing process. After that aged seed were invigorated with hormones i.e., hormonal seed priming. Result: Our finding revealed that the aged seed treated with gibberellic acid and salicylic acid enhanced the activity of antioxidant enzymes which help in seed germination.