Effect of growth factor on in-vitro maturation of porcine oocyte

Abstract

The present study was aimed to evaluate the beneficial effect of different growth factors on in-vitro maturation of porcine oocytes. Ovaries were collected from a local abattoir immediately after slaughter of the animals and transported to the laboratory. A total of 618 type A and type B oocytes were cultured in TCM-199 containing additives with PMSG and hCG for the first 22 hrs and without hormones for subsequent 22 hrs of incubation at 39o C under 5 per cent CO2 level and 90-95 per cent humidity. The effects of supplementation of different growth factors viz., EGF, IGF-I and EGF + IGF-I in the medium were studied. The rate of oocytes with cumulus cells expansion was significantly higher (P less than 0.01) when growth factors were added as compared to control but it did not differ significantly between growth factors. The rate of nuclear maturation of oocytes was significantly higher (P less than 0.01) as compare to control for EGF and EGF + IGF-I but not for IGF-I. There was no significant difference in the rate of oocytes with nuclear maturation between the growth factors studied. It can be concluded from the present study that addition of EGF, IGF-I or EGF + IGF and additives along with hormones (PMSG and hCG for first 20-22 hrs) in TCM-199 Medium gives optimum in-vitro maturation rates in porcine oocytes.