Molecular characterization of lingual antimicrobial peptide gene of local cattle (Bos indicus) of Assam and insilco designing of antimicrobial peptides

Abstract

Mammalian defensin is the one of the important antimicrobial peptides expressed by different epithelial lining of the living organisms. Present study was undertaken to characterize the lingual antimicrobial peptide (LAP) gene of Assam local cattle (Bos indicus ) for insilco designing of peptide for synthesis of novel antimicrobial agents. RNA was isolated from the tongue epithelial of Bos indicus and reverse transcribed with specific primer. The amplified PCR product was purified, cloned and sequenced. The size of the PCR product was 230 bp and cloned cDNA after sequencing revealed the open reading frame (ORF) of 195 bases. The total number of predicted amino acid in the peptide was 64. Aligned amino acid sequences of Bos indicus LAP showed six conserved cysteine residues at different positions. The mature peptide of local cattle LAP had six (6) arginine, three (3) lysine, three (3) proline and one (1) histidin residues. Support vector machine algorithms showed the antimicrobial potency of different segments of the mature peptide and out of them four different most potent peptides were designed. From the present study, it can be concluded that the mature domain of the Bos indicus lingual antimicrobial peptide (LAP) may be use as template for synthesis of new antimicrobial agents.