Comparative Expression Profile of RFRP-3 in Hypothalamus and Testis of Adult Rats

Abstract

Background: The present study focused on the localization and comparative expression analysis of RFRP-3 gene in the hypothalamus and testis of adult male rats. Methods: Brain and testis tissue samples were collected from adult Wister rats and used for immunohistochemistry for identification and localization RFRP-3 producing neurons. The expression level of the RFRP-3 gene was assessed by Real-time PCR and compared with muscle tissue. Result: The results confirmed the presence of RFRP-3 secreting neurons in the hypothalamus and in the testes. Scattered RFRP-3 in cell bodies was identified primarily in the VMH, DMH and surrounding the third ventricle of the brain. In testis, RFRP-3 was detected in Leydig cells, Sertoli cells and germ cells. The expression level of RFRP-3 gene in the hypothalamus was observed to be significantly higher in hypothalamus than in testicular tissue (p less than 0.01) of adult rats.