Effects of Time Specific Supplementation of Insulin Like Growth Factor-I (IGF-I) on in vitro Development of Sheep Preantral Follicles
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Published:2021-07-19
Issue:Of
Volume:
Page:
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ISSN:0976-0555
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Container-title:Indian Journal of Animal Research
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language:
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Short-container-title:IJAR
Author:
Pathipati Deepa,Chigurupati Srinivasa Prasad,Kumar A.V.N. Siva,Kumari B. Punya,Kumar R.V. Suresh
Abstract
Background: In in vitro culture systems IGF-I was known to promote follicular maturation, granulosa cell proliferation and increase overall cellular function. The expression patterns of developmentally important markers for IGF-I are expressed differentially during the transition of preantral follicles to the ovulatory stage. Therefore, we aimed to investigate the influence of time specific addition of Insulin like growth factor-I (IGF-I) on in vitro development of sheep preantral follicles (PFs’).
Methods: Mechanically isolated sheep preantral follicles were cultured for six days either in TCM 199B or standard medium supplemented with IGF-I at different points during the culture period and were subsequently subjected to in vitro maturation (IVM) for additional 24hrs to evaluate different follicular development parameters. Conclusion: Based on the results, it is concluded that: 1) IGF-I supplementation was needed during early rather than later stages of culture period, therefore, role of IGF-I in time/stage specific manner in cultured preantral follicles is confirmed. 2) Supplementation of TCM 199B with IGF-I simultaneously for the first two days followed by TCM 199B alone without any growth factor (s) in later days (Treatment 1) supported better development of PFs’ in vitro.
Publisher
Agricultural Research Communication Center
Subject
General Veterinary,Animal Science and Zoology