Comparative Evaluation of Apoptotic Effects of Vincristine, Cisplatin and Their Hydrogel Scaffolds on HeLa Cell Line

Abstract

The present study has been undertaken for comparative evaluation of apoptotic effects of vincristine, cisplatin and their hydrogel scaffolds on HeLa cell line. Apoptosis can be visualized as a ladder pattern due to DNA cleavage by the activation of a nuclear endonuclease by standard agarose gel electrophoresis. Results showed the formation of the DNA ladder in gel electrophoresis by induction of apoptosis in HeLa cell line treated with 20 µg/ml of vincristine alone, 20 µg/ml cisplatin alone and their scaffolds, a typical ladder pattern of internucleosomal fragmentation was observed in cells after 24 hrs treatment. DNA ladder assay of genomic DNA from HeLa cells was isolated and subjected to electrophoresis in agarose gel (1.8%) and 1kb DNA ladder. DNA fragments were visualized under a UV trans-illuminator and compared with a standard marker. On the basis of the parameters studied, it was concluded that the formation of the DNA ladder in gel electrophoresis by induction of apoptosis in HeLa cell line treated with vincristine, cisplatin alone and their scaffolds showed best fragmentation in vincristine and cisplatin, however, their scaffolds are moderately effective when used in appropriate dose on HeLa cell line.