PCR-RFLP Analysis of Single Nucleotide Polymorphism (SNP) C-2402T at the Promoter Region of Prolactin Gene and its Association with Positively Correlated Production Traits in White Leghorn Chicken

Abstract

Background: The avian prolactin gene is highly conserved, located on chromosome number 2 and most sequence polymorphisms occurs in the 5’ flanking region, 3’ flanking region, and the coding region of signal peptide. The present study was aimed at the identification of SNP C-2402T of prolactin gene and its association with production traits in White Leghorn chicken. Methods: A total of 200 birds of White Leghorn were selected from All India Co-ordinated Research Project on Poultry improvement (AICRP) farm, Mannuthy. Genomic DNA was isolated from venous blood. Polymerase chain reaction (PCR) followed by restriction fragment length polymorphism (RFLP) analysis was done to identify the SNP C-2402T of prolactin gene. Result: All the birds were observed with the same genotype CC and the frequency of the C allele was one.