Association of single nucleotide polymorphisms of PPP1R11 gene with conception rate in Karan Fries bull

Abstract

Bull fertility is an important component of reproductive efficiency in dairy cattle. PPP1R11 is a potent candidate gene for bull fertility being involved in the pathway of spermatogenesis. Present investigation was carried out in twenty Karan Fries (KF) bull to identify SNPs of PPP1R11 gene and their association with conception rate. Genomic DNA was extracted from frozen semen straws by phenol-chloroform method. Primer pair targeted to exonic II region of PPP1R11 gene was designed using Primer 3.1 software. PCR reactions were carried out in thermal cycler after optimizing. The PCR product was checked on 1.5% horizontal agarose gel electrophoresis to verify the amplification of target region. Samples were sequenced and SNPs were identified using multiple alignment programme with respect to reference sequence and visual inspection of chromatograms. Sequencing results revealed only two SNPs [G28708846A, T28708860C] at exonic II region of PPP1R11 gene in KF bulls. Each bull was genotyped with respect to each of the two identified SNPs. Association of SNP genotype with conception rate was analyzed using least square ANOVA taking genotypes as fixed effect. The result indicated that, only one SNP [T28708860C] had significant (p>0.01) effect on conception rate. The bulls with TT genotype revealed significantly (p>0.01) highest conception rate (53.20±2.96%) followed by bulls with TC (43.12±2.39%) and the lowest in bulls with CC (34.59± 2.28%) genotypes. The results of present finding suggested that association of SNPs with conception rate after validation on large population might be useful in marker assisted selection for selection of bull with high fertility in future.