Effect of Centrifugation Regime on Cryopreservation of Beetal Buck Semen

Abstract

Background: Goat semen has phospholipase A enzyme which makes removal of seminal plasma an important step in its preservation. This study was aimed to standardize a centrifugation regime for cryopreservation of Beetal buck semen. Methods: A total of 36 pooled semen ejaculates were used for the experiment where initially 27 pooled ejaculates were utilized to find out the best time period out of 5, 8 and 11 minutes for each g-force viz., 700 x g, 1100 x g and 1400 x g using 9 pooled ejaculates per g-force. The remaining 9 pooled ejaculates were employed to find out the best centrifugation regime amongst the best time period for each g-force (8 minutes for 700 x g, 8 minutes for 1100 x g and 5 minutes for 1400 x g). Result: The percentage of sperm motility, live sperm, intact acrosome and HOST-reacted sperm differed significantly (P less than 0.0001) between time periods at 700 x g, 1100 x g and 1400 x g. The sperm parameters were significantly (P less than 0.05) higher at 1400 x g for 5 minutes and 1100 x g for 8 minutes than at 700 x g for 8 minutes. In conclusion, adoption of a high centrifugation force for a short duration of time for washing could improve the quality of frozen spermatozoa.