29-kDa: A Potential Candidate for Anti-Tick Vaccine Antigen Source as Immunogenic and Stage Reactive Targeting Hard-Bodied Hyalomma Ticks Ixodidae
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Published:2020-02-22
Issue:of
Volume:
Page:
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ISSN:0976-0555
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Container-title:Indian Journal of Animal Research
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language:
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Short-container-title:IJAR
Author:
Kamran Kashif,Villagra Cristian A,Iqbal Asim,Kakar Asmatullah,Schapheer Constaza,Taj Muhammad Kamran,Ali Abid,Siddiqui Saima
Abstract
The objective of present study is to develop a vaccine against Hyalomma hard-bodied tick and to analyze relevant experimental data on immunized indigenous horse breed Morna. Montanide (ISA-50) adjuvant-based vaccine induced significantly (pandlt;0.02) higher antibody titre through intradermal route with 99.98% vaccine efficacy. Humoral response was determined though indirect ELISA; where peak level of serum antibody was recorded after six weeks interval of post-immunization. Most of hematology and biochemical parameters remained consistent to normal reference values. Our report also indicates a significant percentage decline in the numbers of engorged ticks, eggs mass, eggs number and increased tick rejection. The animal travel history can promote tick burden and is a potential risk factor. Based on biological and hematological parameters, it is possible to conclude that 29-kDa antigen can be used as an effective antigen vaccine candidate to control tick infestation detected through humoral response.
Publisher
Agricultural Research Communication Center
Subject
General Veterinary,Animal Science and Zoology