Seroprevalence and Molecular Detection of Bovine Brucellosis

Abstract

Background: Brucellosis is one of the major zoonotic problems that exist worldwide. Brucellosis is clinically characterized by metritis, mastitis, repeat breeding, abortion in the last trimester of pregnancy, retention of placenta and reduced milk production in the female whereas epididymitis, orchitis and sterility in male. In humans can be highly variable, ranging from nonspecific, flu-like symptoms to undulant fever, arthritis, orchitis and epididymitis. Methods: A total of 567 bovine serum samples was taken from four districts of Brij region of UP. All the samples were processed to detection of prevalence of brucellosis by RBPT, STAT ELISA and confirmation of genes bcsp31, 16SrRNA, omp2 and IS711 by PCR. Result: The prevalence of brucellosis was found to be 07.93% (31/391), 08.69% (34/391) and 10.74% (42/391) shows positive by RBPT, STAT and I- ELISA respectively. In buffalo Out of 176 tested serum sample the seroprevalence was found to be 09.66% (17/176), 10.79% (19/176) and 12.5% (22/176) positive by RBPT, STAT and I- ELISA respectively. Out of 567 samples 18 were positive for Brucella genus specific gene. The higher prevalence of the disease in this region increases the risk of zoonotic transmission and it implies a serious threat to the human population as well as the huge impact on economy due to loss of productivity as well as loss of livestock population.