Predicted and In vitro Effect of Deltamethrin on Bone Marrow Progenitors

Abstract

Background: Deltamethrin, a type 2 pyrethroid, reported toxicity said to have atoxic effect upon chronic exposure on the immune system. Since bone marrow is the source of immune cells, it is appropriate to test impact of the widely used Deltamethrin on BMSCs (Bone marrow stem cells). It was found that long term exposure to these toxic pesticides used for domestic and agricultural purposes showed to cause severe hematotoxicity and degenerative marrow diseases, precisely hypoplastic bone marrow failure condition in mammals. The present study analysed the less explored predicted effects of Deltamethrin on blood stem cells through CD45 receptor and virtual effects on cultured cells.Methods: The effect of deltamethrin on bone marrow-derived stem cells at different concentrations and time periods was analysed. To further understand the molecular basis of how these chemicals affect the protein-ligand binding, molecular docking studies were conductedResult: Our results on iGEMDOCK 2.1 software used to dock CD45 receptors with DLM and the binding mode indicated that the bound ligand and the active site of CD45 had a good binding score of -68.04 and formed the five H bonds with the amino acids residues of CD45. It is found from the current study that there is an increase in cytotoxicity with increased exposure to deltamethrin. Further studies can be done to note the precise target of such agents to quickly underst and and control the effect of such drugs on a safer lifestyle.