In vivo anti-inflammatory activity of M116, one extract obtained from the marine bacterium Bacillus amyloliquefaciens
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Published:2023-07-01
Issue:4
Volume:11
Page:651-661
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ISSN:0719-4250
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Container-title:Journal of Pharmacy & Pharmacognosy Research
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language:
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Short-container-title:J Pharm Pharmacogn Res
Author:
Hernández-Balmaseda IvonesORCID,
López Lezcano FernandoORCID,
Cuellar Duarte Cindel,
Garrido-Suárez Bárbara B.ORCID,
Ortiz Guilarte EudalysORCID,
Fernández Pérez Miguel D.ORCID,
Rodeiro Guerra IdaniaORCID
Abstract
Context: Marine organisms are sources of compounds with anti-inflammatory activity, many of them derived from the secondary metabolites produced by microorganisms. Aims: To evaluate the possible anti-inflammatory effect of M116, an extract obtained by fermentation from the CBM-116 strain of the marine bacterium Bacillus amyloliquefaciens, which was isolated from sediments of the southern coast of the Cuban shelf. Methods: The oral single and repeated different doses of the CBM-116 were evaluated for their ability to ameliorate edema using two in vivo experimental inflammation models: croton oil-induced atrial acute edema and cotton pellets-induced chronic granuloma, both in male Balb/c mice. The systemic production of redox biomarkers after repeated doses in the chronic inflammation model was also tested. Results: A single application of M116 (50-200 mg/kg, 10 mL/kg, p.o.) decreases croton oil-induced acute inflammation in a dose-dependent manner. Single and repeated doses of extract (100-400 mg/kg, p.o.) also were able to inhibit chronic inflammation during both, transudative and proliferative phases of the inflammatory process. This effect was associated with the systemic reduction of oxidative stress. Conclusions: M116 showed anti-inflammatory activity in the context of acute and chronic inflammation associated with its antioxidant mechanisms, which suggest the potential of the marine bacterium Bacillus amyloliquefaciens as a source of new products with biomedical application.
Publisher
Garval Editorial Ltda.
Subject
Drug Discovery,Pharmaceutical Science,Pharmacology,Pharmacy,Complementary and alternative medicine