A dual‐function SNF2 protein drives chromatid resolution and nascent transcript removal in mitosis

Author:

Carmo Catarina1ORCID,Coelho João1ORCID,Silva Rui D2,Tavares Alexandra1ORCID,Boavida Ana1,Gaetani Paola1ORCID,Guilgur Leonardo G1ORCID,Martinho Rui Gonçalo23ORCID,Oliveira Raquel A14ORCID

Affiliation:

1. Instituto Gulbenkian de Ciência Oeiras Portugal

2. Algarve Biomedical Center Research Institute (ABC‐RI) and Faculty of Medicine and Biomedical Sciences (FMCB) Universidade do Algarve Faro Portugal

3. Department of Medical Sciences (DCM) and Institute for Biomedicine (iBiMED) Universidade de Aveiro Aveiro Portugal

4. Católica Biomedical Research Centre, Católica Medical School Universidade Católica Portuguesa Lisbon Portugal

Abstract

AbstractMitotic chromatin is largely assumed incompatible with transcription due to changes in the transcription machinery and chromosome architecture. However, the mechanisms of mitotic transcriptional inactivation and their interplay with chromosome assembly remain largely unknown. By monitoring ongoing transcription in Drosophila early embryos, we reveal that eviction of nascent mRNAs from mitotic chromatin occurs after substantial chromosome compaction and is not promoted by condensin I. Instead, we show that the timely removal of transcripts from mitotic chromatin is driven by the SNF2 helicase‐like protein Lodestar (Lds), identified here as a modulator of sister chromatid cohesion defects. In addition to the eviction of nascent transcripts, we uncover that Lds cooperates with Topoisomerase 2 to ensure efficient sister chromatid resolution and mitotic fidelity. We conclude that the removal of nascent transcripts upon mitotic entry is not a passive consequence of cell cycle progression and/or chromosome compaction but occurs via dedicated mechanisms with functional parallelisms to sister chromatid resolution.

Publisher

EMBO

Subject

Genetics,Molecular Biology,Biochemistry

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