Affiliation:
1. Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Sciences Nanjing Normal University Nanjing China
2. Department of Radiotherapy, Taikang Xianlin Drum Tower Hospital Nanjing University Nanjing China
3. School of Basic Medical Sciences Nanjing Medical University Nanjing China
4. Department of Bioinformatics, School of Biomedical Engineering and Informatics Nanjing Medical University Nanjing China
5. Department of Oncology, Nanjing First Hospital Nanjing Medical University Nanjing China
Abstract
AbstractHomologous recombination (HR), a form of error‐free DNA double‐strand break (DSB) repair, is important for the maintenance of genomic integrity. Here, we identify a moonlighting protein, glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH), as a regulator of HR repair, which is mediated through HDAC1‐dependent regulation of RAD51 stability. Mechanistically, in response to DSBs, Src signaling is activated and mediates GAPDH nuclear translocation. Then, GAPDH directly binds with HDAC1, releasing it from its suppressor. Subsequently, activated HDAC1 deacetylates RAD51 and prevents it from undergoing proteasomal degradation. GAPDH knockdown decreases RAD51 protein levels and inhibits HR, which is re‐established by overexpression of HDAC1 but not SIRT1. Notably, K40 is an important acetylation site of RAD51, which facilitates stability maintenance. Collectively, our findings provide new insights into the importance of GAPDH in HR repair, in addition to its glycolytic activity, and they show that GAPDH stabilizes RAD51 by interacting with HDAC1 and promoting HDAC1 deacetylation of RAD51.
Funder
National Natural Science Foundation of China
Natural Science Research of Jiangsu Higher Education Institutions of China
Publisher
Springer Science and Business Media LLC
Subject
Genetics,Molecular Biology,Biochemistry