Glyoxal as an alternative fixative to formaldehyde in immunostaining and super‐resolution microscopy

Author:

Richter Katharina N12,Revelo Natalia H1ORCID,Seitz Katharina J13,Helm Martin S13,Sarkar Deblina4,Saleeb Rebecca S5,D'Este Elisa6,Eberle Jessica7,Wagner Eva89,Vogl Christian1011ORCID,Lazaro Diana F1213,Richter Frank314,Coy‐Vergara Javier15,Coceano Giovanna16,Boyden Edward S17,Duncan Rory R5,Hell Stefan W6,Lauterbach Marcel A7,Lehnart Stephan E89,Moser Tobias1011ORCID,Outeiro Tiago F1213ORCID,Rehling Peter1418,Schwappach Blanche15,Testa Ilaria16,Zapiec Bolek19,Rizzoli Silvio O12ORCID

Affiliation:

1. Department of Neuro‐ and Sensory Physiology University of Göttingen Medical Center Göttingen Germany

2. Cluster of Excellence Nanoscale Microscopy and Molecular Physiology of the Brain Göttingen Germany

3. International Max Planck Research School Molecular Biology Göttingen Germany

4. MIT Media Lab

5. Edinburgh Super‐Resolution Imaging Consortium Institute of Biological Chemistry, Biophysics, and Bioengineering Heriot‐Watt University Edinburgh UK

6. Department of NanoBiophotonics Max‐Planck‐Institute for Biophysical Chemistry Göttingen Germany

7. Department of Neural Systems Max‐Planck‐Institute for Brain Research Frankfurt am Main Germany

8. Heart Research Center Göttingen Department of Cardiology & Pulmonology University Medical Center Göttingen Göttingen Germany

9. German Center for Cardiovascular Research (DZHK) Site Göttingen

10. Institute for Auditory Neuroscience and InnerEarLab University Medical Center Göttingen Göttingen Germany

11. Max‐Planck‐Institute for Experimental Medicine Auditory Neuroscience Group Göttingen Germany

12. Department of Experimental Neurodegeneration Center for Nanoscale Microscopy and Molecular Physiology of the Brain Center for Biostructural Imaging of Neurodegeneration University Medical Center Göttingen Göttingen Germany

13. Max‐Planck‐Institute for Experimental Medicine Göttingen Germany

14. Department of Cellular Biochemistry University Medical Center Göttingen Göttingen Germany

15. Department of Molecular Biology University Medical Center Göttingen Göttingen Germany

16. Department of Applied Physics and Science for Life Laboratory KTH Royal Institute of Technology Stockholm Sweden

17. Departments of Brain and Cognitive Science and Biological Engineering MIT Media Lab and McGovern Institute Cambridge MA USA

18. Max‐Planck‐Institute for Biophysical Chemistry Göttingen Germany

19. Max Planck Research Unit for Neurogenetics Frankfurt am Main Germany

Funder

Seventh Framework Programme

Deutsche Forschungsgemeinschaft

H2020 European Research Council

Boehringer Ingelheim Fonds

Research Executive Agency

European Molecular Biology Organization

Nederlandse Organisatie voor Wetenschappelijk Onderzoek

Deutsches Zentrum für Herz-Kreislaufforschung

United States - Israel Binational Science Foundation

Publisher

EMBO

Subject

General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,Molecular Biology,General Neuroscience

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