Evaluation of Mineral Contents and Antioxidant Ability of Black Pepper Seed Oil by Soxhlet and Cold Pressing

Abstract

In present study, the evaluation of mineral contents of black pepper seeds is determined by atomic absorption spectrometry. The mineral analysis of the Atomic Absorption Spectrometry (AAS) of black pepper is consist of Manganese (Mn) 2.282 ± 0.01 µg/ml, Magnesium (Mg) 8.148 ± 0.08 µg/ml, Copper (Cu) 0.27 ± 0.005 µg/ml, Zinc (Zn) 0.593 ± 0.01 µg/ml and Iron (Fe) 0.89 ± 0.005 µg/ml. The efficiency and stability of natural antioxidant in black pepper seed oil is determined by reducing power (FRAP). Free radicals present in oils and fats are responsible for harmful for the body cell. The stability of natural antioxidant of black pepper seed oil and their significance at different concentrations is calculated by One Way ANOVA Test. In present study, the result showed that the increased concentration of black pepper seed oil by solvent soxhlet extraction and cold pressing (25 µg/ml - 100 µg/ml) has positive correlation with reducing power assay. The result is showed that black pepper oil has ability to remove toxic compounds produced in the body and helpful maintain the body cells. In addition, the extraction methods can helpful for the improvement in the preservation of important nutrients which may be sensitive to thermal heat. Cold pressing is useful method as it can exclude the use of heat as well as organic solvent in soxhlet method.