An In silico Approach for Structural and Functional Annotation of Uncharacterized Protein Rv0986 present in Mycobacterium tuberculosis

Abstract

Tuberculosis (TB) is an ancient infectious disease caused by Mycobacterium tuberculosis (MTB). MTB is a human pathogen. Surprisingly, TB has become the top disease for its death rate worldwide. The uncharacterized protein Rv0986 is closely related to the transporters of the ATP-binding cassette domain, therefore, take part in the export of macrolide as well as a lipoprotein. Furthermore, it is associated with cell division protein. Hence, the protein has a significant role in mycobacterial infection. But, so far, the uncharacterized protein Rv0986 is not elaborated. As a result, in this study, the structural and functional annotation of the protein is described through in silico approach. The predicted tertiary structures of the protein generated by Swiss Model, Modeller, and Phyre2, and documented by the Ramachandran Plot analysis with PROCHECK, Verify 3D, and Swiss-Model Interactive Workplace. Z-score also applied for the overall structural assessment. This study will unleash the importance of the uncharacterized protein present in MTB, therefore, it provides an opportunity for drug and vaccine targeting against infection by MTB.