The Application of Resonance Light Scattering Technique for the Determination of Tinidazole in Drugs

Abstract

A resonance light scattering technique to determine tinidazole in drugs was developed by tetraphenylboron sodium (TPB). Tinidazole was found to bindB(C6H5)4−anion and transformed to tinidazole-TPB aggregate which displayed intense resonance scattering light. Effects of factors such as wavelength, acidity, stabilizers, and interferents on the RLS of tinidazole TPB were investigated in detail. The RLS intensity of the tinidazole-TPB suspension was obtained in sulfuric acid solution(pH=1.44). The resonance scattering light intensity at the maximum RLS peak of 569.5 nm was linear to the concentration of tinidazole in the range of 10.0–30.0μgmL−1with a detection limit of 5.0μgmL−1. Good results were also obtained with the recovery range of 95.13–106.76%. The method was applied to determine tinidazole in injections and tablets, showing high sensitivity and accuracy compared with the high performance liquid chromatography method (HPLC) according to Chinese Pharmacopoeia.