Biocontrol ofPhytophthora infestans, Fungal Pathogen of Seedling Damping Off Disease in Economic Plant Nursery

Abstract

This research aims to control Seedling damping off disease in plants by using antagonistic actinomycetes against the causative fungi.Phytophthora infestanswas isolated from the infected tomato plant seedling obtained from an economic plant nursery in Amphoe Pak Chong, Nakhon Ratchasima Province, Thailand. The chitinolyticStreptomyces rubrolavendulaeS4, isolated from termite mounds at the grove of Amphoe Si-Sawat, Kanchanaburi Province, Thailand, was proven to be the most effective growth inhibition of fungal pathogens tested on potato dextrose agar. Tomato and chili seedlings that colonized with antagonisticS. rubrolavendulaeS4 were grown inP. infestansartificial inoculated peat moss. Percents of noninfested seedling in fungal contaminated peat moss were compared to the controls with uninoculated peat moss. InP. infestanscontaminated peat moss, the percents of survival of tomato and chili seedling were significantly increased () from 51.42 to 88.57 and 34.10 to 76.71 for theS. rubrolavendulaeS4 treatment, respectively. TheS. rubrolavendulaeS4 also showed high efficiency equivalent to fungicide, metalaxyl with no significant difference (). It was clearly demonstrated thatS. rubrolavendulaeS4 can prevent the tomato and chili seedling damping off disease in economic plant nurseries.