Development of an Amperometric Glucose Biosensor Based on the Immobilization of Glucose Oxidase on the Se-MCM-41 Mesoporous Composite

Author:

Yusan Sabriye12,Rahman Mokhlesur M.3ORCID,Mohamad Nasir3,Arrif Tengku M.3,Latif Ahmad Zubaidi A.4,M. A. Mohd Aznan5,Wan Nik Wan Sani B.6ORCID

Affiliation:

1. Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, 81310 Johor Bahru, Johor, Malaysia

2. Institute of Nuclear Science, Ege University, Bornova, 35100 Izmir, Turkey

3. Institute for Community Development & Quality of Life (i-CODE), Universiti Sultan Zainal Abidin, 21300 Kuala Nerus, Terengganu, Malaysia

4. Faculty of Medicine, Universiti Sultan Zainal Abidin, 21300 Kuala Nerus, Terengganu, Malaysia

5. Faculty of Medicine, International Islamic University Malaysia, 25200 Kuantan, Malaysia

6. School of Ocean Engineering, Universiti Malaysia Terengganu, 21300 Kuala Nerus, Terengganu, Malaysia

Abstract

A new bioenzymatic glucose biosensor for selective and sensitive detection of glucose was developed by the immobilization of glucose oxidase (GOD) onto selenium nanoparticle-mesoporous silica composite (MCM-41) matrix and then prepared as a carbon paste electrode (CPE). Cyclic voltammetry was employed to probe the catalytic behavior of the biosensor. A linear calibration plot is obtained over a wide concentration range of glucose from 1 × 10−5 to 2 × 10−3 M. Under optimal conditions, the biosensor exhibits high sensitivity (0.34 µA·mM−1), low detection limit (1 × 10−4 M), high affinity to glucose (Km = 0.02 mM), and also good reproducibility (R.S.D. 2.8%, n=10) and a stability of about ten days when stored dry at +4°C. Besides, the effects of pH value, scan rate, mediator effects on the glucose current, and electroactive interference of the biosensor were also discussed. As a result, the biosensor exhibited an excellent electrocatalytic response to glucose as well as unique stability and reproducibility.

Funder

Universiti Teknologi Malaysia

Publisher

Hindawi Limited

Subject

Computer Science Applications,Instrumentation,General Chemical Engineering,Analytical Chemistry

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