Enhancing Lysostaphin Thermostability and Utilizing Lysostaphin‐Producing Probiotics to Combat Staphylococcus aureus in Milk

Author:

Zhang ShuhangORCID,Wen KangshuORCID,Song WenxiaORCID,Li QingbinORCID,Qi QingshengORCID,Lu XuemeiORCID

Abstract

The emergence and spread of methicillin‐resistant Staphylococcus aureus (MRSA) is a major cause of bacterial infections in healthcare. Lysostaphin, a Zn2+‐dependent endopeptidase, hydrolyzes the glycine (G)‐linked pentapeptide bridge in the pathogen Staphylococcus aureus. In this study, the stability of lysostaphin was improved by engineering the addition of disulfide bonds around the protein’s Zn2+ binding site through molecular dynamics simulation. Further mutagenesis to add polyG at the C‐terminus yielded combination variant 319/252‐G3, which was 2.6‐fold more stable than lysostaphin after 30 min at 60°C. Optimization of the C‐terminal glycine number produced variant 319/252‐G6, which retained 67.3% of its antimicrobial activity after 30 min at 70°C, and could be resistant to milk pasteurization conditions. Based on this, a probiotic strain of Escherichia coli Nissle 1917 to constitutively express and secrete lysostaphin from a mutated lac operator was engineered, resulting in effective control and prevention of S. aureus contamination in milk. One possible biotechnological application to utilize lysostaphin and probiotic strains can be used in animal feed and food additives for the prevention and treatment of S. aureus infections.

Publisher

Wiley

Reference46 articles.

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3