A Complete Molecular Diagnostic Procedure for Applications in Surveillance and Subtyping of Avian Influenza Virus-Reference-Cited by-同舟云学术

A Complete Molecular Diagnostic Procedure for Applications in Surveillance and Subtyping of Avian Influenza Virus

Published:2014 Issue: Volume:2014 Page:1-7
ISSN:2314-6133
Container-title:BioMed Research International
language:en
Short-container-title:BioMed Research International

Author:

Tseng Chun-Hsien¹,Tsai Hsiang-Jung¹²,Chang Chung-Ming³

Affiliation:

1. Animal Health Research Institute, Danshui, Taipei 25158, Taiwan

2. Graduate Institute of Veterinary Medicine, College of Bioresource and Agriculture, National Taiwan University, No. 1, Section 4, Roosevelt Road, Taipei 106, Taiwan

3. Research Center for Emerging Viral Infections, Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, Kwei-Shan, Taoyuan 333, Taiwan

Abstract

Introduction. The following complete molecular diagnostic procedure we developed, based on real-time quantitative PCR and traditional PCR, is effective for avian influenza surveillance, virus subtyping, and viral genome sequencing.Method. This study provides a specific and sensitive step-by-step procedure for efficient avian influenza identification of 16 hemagglutinin and 9 neuraminidase avian influenza subtypes.Result and Conclusion. This diagnostic procedure may prove exceedingly useful for virological and ecological advancements in global avian influenza research.

Funder

National Science Council

Publisher

Hindawi Limited

Subject

General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

Link

http://downloads.hindawi.com/journals/bmri/2014/653056.pdf

Reference21 articles.

1. Crossing the Species Barrier: The Threat of an Avian Influenza Pandemic

2. The Evolving Threat of Influenza Viruses of Animal Origin and the Challenges in Developing Appropriate Diagnostics

3. Towards improved influenza A virus surveillance in migrating birds