Characterization of Acetylation of Histone H3 at Lysine 9 in the Trigeminal Ganglion of a Rat Trigeminal Neuralgia Model

Author:

Wei Wenbin12345ORCID,Liu Yuemin12345ORCID,Qiu Yating12345ORCID,Chen Minjie12345ORCID,Wang Yiwen12345ORCID,Han Zixiang12345ORCID,Chai Ying12345ORCID

Affiliation:

1. Department of Oral Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China

2. College of Stomatology, Shanghai Jiao Tong University, Shanghai, China

3. National Center for Stomatology, Shanghai, China

4. National Clinical Research Center for Oral Diseases, Shanghai, China

5. Shanghai Key Laboratory of Stomatology, Shanghai, China

Abstract

Trigeminal neuralgia (TN) is a chronic neuropathic pain disorder characterized by spontaneous and elicited paroxysms of electric-shock-like or stabbing pain in a region of the face. The epigenetic regulation of TN is still obscure. In current study, a rat TN model subject to carbamazepine (CBZ) treatment was established, and transcriptome- and genome-scale profiling of H3K9ac and HDAC3 was performed by RNA-seq and ChIP-seq. We observed that H3K9ac levels in the trigeminal ganglion were lower in the TN rats compared with those in the control, and CBZ treatment led to recovery of H3K9ac levels. Further, we found that HDAC3 was overactivated, which interfered with H3K9 acetylation due to higher phosphorylation in TN compared with that in the control. Finally, the phosphokinase leucine-rich repeat kinase 2 (LRRK2) was demonstrated to contribute to HDAC3 activity via the MAPK signaling pathway. Taken together, we identified a regulatory mechanism in which the phosphate groups transferred from activated ERK and LRRK2 to HDAC3 caused genome-scale deacetylation at H3K9 and resulted in the silencing of a large number of genes in TN. The kinases or important enzymes within this regulatory axis may represent important targets for TN therapy and prevention.

Funder

Science and Technology Commission of Shanghai Municipality

Publisher

Hindawi Limited

Subject

Cell Biology,Aging,General Medicine,Biochemistry

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