Pharmacological Basis for Traditional Use of theLippia thymoides

Author:

Silva Fabrício Souza12,Menezes Pedro Modesto Nascimento1,Sá Pedro Guilherme Souza de1,Oliveira André Luís de Santana3,Souza Eric Alencar Araújo1,Bamberg Vinicius Martins1,de Oliveira Henrique Ribeiro1,Oliveira Sheilla Andrade de4,Araújo Roni Evêncio e4,Uetanabaro Ana Paula Trovatti5,Silva Tânia Regina dos Santos6,Almeida Jackson Roberto Guedes da Silva127,Lucchese Angélica Maria23

Affiliation:

1. Núcleo de Estudos e Pesquisas em Plantas Medicinais, Colegiado de Ciências Farmacêuticas, Universidade Federal do Vale do São Francisco, Avenida José de Sá Maniçoba, s/n, Centro, 56304-917 Petrolina, PE, Brazil

2. Programa de Pós-Graduação em Biotecnologia, Departamento de Ciências Biológicas, Universidade Estadual de Feira de Santana, Avenida Transnordestina, s/n, Novo Horizonte, 44036-900 Feira de Santana, BA, Brazil

3. Laboratório de Química de Produtos Naturais e Bioativos, Departamento de Ciências Exatas, Universidade Estadual de Feira de Santana, Avenida Transnordestina, s/n, Novo Horizonte, 44036-900 Feira de Santana, BA, Brazil

4. Laboratório de Imunopatologia e Biologia Molecular, Departamento de Centro de Pesquisa Aggeu Magalhães, Fundação Oswaldo Cruz, Avenida Professor Moraes Rego, s/n, Campus da UFPE, Cidade Universitária, 50670-420 Recife, PE, Brazil

5. Laboratório de Microbiologia da Agroindústria, Universidade Estadual de Santa Cruz, Ilhéus, BA, Brazil

6. Departamento de Ciências Biológicas, Universidade Estadual de Feira de Santana, Feira de Santana, BA, Brazil

7. Programa de Pós-Graduação em Recursos Naturais do Semi-Árido, Universidade Federal do Vale do São Francisco, Avenida José de Sá Maniçoba s/n, Centro, 56304-917 Petrolina, PE, Brazil

Abstract

The aim of this study was to evaluate crude extracts and fractions from leaves and stems ofLippia thymoidesand to validate their use in folk medicine.In vitroantioxidant and antimicrobial activities andin vivowound healing in rats, baker yeast-induced fever in young rats, and acute oral toxicity in mice assays were realized. The crude extracts and their dichloromethane and ethyl acetate fractions had potent radical-scavenging activity against the DPPH but were not effective in theβ-carotene bleaching method. The dichloromethane fraction from the leaves extract showed the broadest spectrum of activity againstS. aureus,B. cereus, andC. parapsilosis. The animals treated with crude extracts showed no difference in wound healing when compared with the negative control group. The crude extract from leaves (1200 mg/kg) has equal efficacy in reducing temperature in rats with hyperpyrexia compared to dipyrone (240 mg/kg) and is better than paracetamol (150 mg/kg). In acute toxicity test, crude extract of leaves fromLippia thymoidesexhibited no mortality and behavioral changes and no adverse effects in male and female mice. This work validates the popular use ofLippia thymoidesfor treating the wound and fever, providing a source for biologically active substances.

Funder

Conselho Nacional de Desenvolvimento Científico e Tecnológico

Publisher

Hindawi Limited

Subject

Complementary and alternative medicine

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