Effects of Electroacupuncture on the Correlation between Serum and Central Immunity in AD Model Animals

Abstract

Objective. The goal was to investigate the connection between neuroinflammation in the brain and serum inflammatory markers as Alzheimer’s disease progressed. We also sought to determine whether electroacupuncture had an effect on inflammatory markers found in blood and other brain regions. Methods. As an animal model for AD, we used senescence-accelerated mouse prone 8 (SAMP8) mice. To examine the effects and probable mechanism of electroacupuncture, we used HE staining, immunofluorescence staining, western blotting, and enzyme-linked immunosorbent assay. Results. Electroacupuncture therapy protected neurons, significantly downregulated the Iba-1 level in the hippocampus ( $p$ value was 0.003), frontal lobe cortex ( $p$ value was 0.042), and temporal lobe cortex ( $p$ value was 0.013) of the AD animal model, all of which had significantly lower levels of IL-6 ( $p$ value was 0.001), IL-1β ( $p$ value was 0.001), and TNF-α ( $p$ value was 0.001) in their serum. Conclusion. The amounts of IL-6, IL-1β, and TNF-α detected in the serum were strongly linked to the levels discovered in the hippocampus and the frontal lobes of the brain, respectively. A better understanding of the electroacupuncture process as well as the course of Alzheimer’s disease and the therapeutic benefits of electroacupuncture may be gained by using biomarkers such as serum inflammatory marker biomarkers.