Interleukin 16- (IL-16-) Targeted Ultrasound Imaging Agent Improves Detection of Ovarian Tumors in Laying Hens, a Preclinical Model of Spontaneous Ovarian Cancer

Author:

Barua Animesh1,Yellapa Aparna2,Bahr Janice M.3,Adur Malavika K.3,Utterback Chet W.4,Bitterman Pincas5,Basu Sanjib6,Sharma Sameer7,Abramowicz Jacques S.89

Affiliation:

1. Departments of Pharmacology, Obstetrics and Gynecology, and Pathology, Rush University Medical Center, Chicago, IL 60612, USA

2. Department of Pharmacology, Rush University Medical Center, Chicago, IL 60612, USA

3. Department of Animal Sciences, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA

4. Poultry Research Farm, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA

5. Departments of Pathology and Obstetrics and Gynecology, Rush University Medical Center, Chicago, IL 60612, USA

6. Department of Preventive Medicine (Biostatistics), Rush University Medical Center, Chicago, IL 60612, USA

7. Departments of Pharmacology, and Obstetrics and Gynecology, Rush University Medical Center, Chicago, IL 60612, USA

8. Department of Obstetrics and Gynecology, Rush University Medical Center, Chicago, IL 60612, USA

9. Department of Obstetrics and Gynecology, Wayne State University, Detroit, MI 48201, USA

Abstract

Limited resolution of transvaginal ultrasound (TVUS) scanning is a significant barrier to early detection of ovarian cancer (OVCA). Contrast agents have been suggested to improve the resolution of TVUS scanning. Emerging evidence suggests that expression of interleukin 16 (IL-16) by the tumor epithelium and microvessels increases in association with OVCA development and offers a potential target for early OVCA detection. The goal of this study was to examine the feasibility of IL-16-targeted contrast agents in enhancing the intensity of ultrasound imaging from ovarian tumors in hens, a model of spontaneous OVCA. Contrast agents were developed by conjugating biotinylated anti-IL-16 antibodies with streptavidin coated microbubbles. Enhancement of ultrasound signal intensity was determined before and after injection of contrast agents. Following scanning, ovarian tissues were processed for the detection of IL-16 expressing cells and microvessels. Compared with precontrast, contrast imaging enhanced ultrasound signal intensity significantly in OVCA hens at early(P<0.05)and late stages(P<0.001). Higher intensities of ultrasound signals in OVCA hens were associated with increased frequencies of IL-16 expressing cells and microvessels. These results suggest that IL-16-targeted contrast agents improve the visualization of ovarian tumors. The laying hen may be a suitable model to test new imaging agents and develop targeted anti-OVCA therapeutics.

Funder

U.S. Department of Defense

Publisher

Hindawi Limited

Subject

General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

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