Antioxidant and Antibacterial Assays onPolygonum minusExtracts: Different Extraction Methods

Abstract

The effect of solvent type and extraction method was investigated to study the antioxidant and antibacterial activity ofPolygonum minus. Two extraction methods were used: a solvent extraction using Soxhlet apparatus and supercritical fluid extraction (SFE). The antioxidant capacity was evaluated using the ferric reducing/antioxidant power (FRAP) assay and the free radical-scavenging capacity of 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The highest polyphenol content was obtained from the medium polarity methanol extract of the leaf portion (645.60 ± 166.68 gallic acid equivalents/100 g (GAEs/100 g)). It also showed the highest antioxidant power for FRAP and DPPH radical inhibition and exhibited the largest inhibition zone in antibacterial activity onBacillus subtilis(Gram+),Staphylococcus aureus(Gram+), andEscherichia coli(Gram−). The phase behavior and aldehyde profiles were further investigated using SFE with different cosolvents. The results indicated that a 50% ethanol-water cosolvent yielded the best aldehyde profiles in the presence of decanal, undecanal, and dodecanal.