Prevalence and Correlation with Clinical Diseases ofHelicobacter pylori cagAandvacAGenotype among Gastric Patients from Northeast China

Abstract

Helicobacter pylori vacAandcagAgenes have significant genetic heterogenicity, resulting in different clinical outcomes. Northeast part of China has reported high prevalence ofH. pyloriinfections and gastric cancer. Hence, we investigated theH. pylori cagAandvacAgenotypes with clinical outcomes in Northeast China. Gastric tissue samples (n=169), chronic gastritis (GIs), gastric ulcer (GU), and gastric cancer (GC) were analysed for 16S rRNAureA,cagA, andcagAgenotypes by PCR. A total of 141 (84%) cases were found positive forH. pyloriby 16S rRNA andureA. GC showed highH. pyloriinfection (93%) compared with GIs (72%) and GU (84%). ThevacAs1am1 was highly found in GC (40%) and GU (36%),vacAs1am2 in GIs (33%),vacAs1bm1 (14%) andvacAs1bm2 (8%) in GU cases, and s2m1 in normal cases (33%), whilevacAs1cm1 showed low frequency in GIs (2%) and GU (3%) and GC showed negative result. The East-AsiancagAstrain was highly observed in GC (43%), as compared to GIs (41%) and GU (20%). The East-AsiancagA/vacAs1am1 was significantly higher in GC (23%) than in GU (22%) and GIs (145) patients. The East-Asian typecagAwithvacAs1a andvacAm1 is the most predominant genotype inH. pyloristrains of Northeast China.