Identification and Characterization of Key Chemical Constituents in Processed Gastrodia elata Using UHPLC-MS/MS and Chemometric Methods

Author:

Ye Xide12ORCID,Wang Yanhong2,Zhao Jianping2,Wang Mei2,Avula Bharathi2,Peng Qiaozhen3,Ouyang Hui1,Lingyun Zhong1,Zhang Jinlian1,Khan Ikhlas A.24ORCID

Affiliation:

1. School of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi 344000, China

2. National Center for Natural Products Research, School of Pharmacy, The University of Mississippi, Jackson, MS 38677, USA

3. School of Computer Information Engineering, Nanchang Hangkong University, Nanchang, Jiangxi 330063, China

4. Division of Pharmacognosy, Department of BioMolecular Sciences, School of Pharmacy, The University of Mississippi, Jackson, MS 38677, USA

Abstract

Gastrodia elata Blume belongs to the Orchidaceae family. G. elata is often processed when used in traditional Chinese medicine (TCM). In the current study, a traditional processing method, known as “Jianchang Bang,” was applied. Steamed and dried (S&D) G. elata was processed with ginger juice for up to 5 days (GEP5D). An UHPLC-MS/MS combined with a chemometric method was developed for the analysis of processed G. elata along with the raw material as well as steamed and dried G. elata. As a result, the primary marker compounds were identified with the aid of TOF-MS and MS/MS analyses. Compared with the raw material of G. elata with GEP5D, three new parishin-type compounds were identified according to their retention time, accurate mass, and fragmentation patterns. The chromatographic peak areas for marker compounds, including S-(gastrodin)-glutathione, S-(4-hydroxybenzylamine)-glutathione, and parishin-type compounds, changed significantly. This result indicated that by applying the “Jianchang Bang” method, changes in chemical composition in G. elata contents were observed. The study also demonstrated that chemometric analysis is helpful in understanding the processing mechanism and will provide scientific support for the clinical application of G. elata.

Funder

National Natural Science Foundation of China

Publisher

Hindawi Limited

Subject

Computer Science Applications,Instrumentation,General Chemical Engineering,Analytical Chemistry

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