Interaction between Chromosomal Protein HMGB1 and DNA Studied by DNA-Melting Analysis-Reference-Cited by-同舟云学术

Interaction between Chromosomal Protein HMGB1 and DNA Studied by DNA-Melting Analysis

Published:2014 Issue: Volume:2014 Page:1-5
ISSN:2314-4920
Container-title:Journal of Spectroscopy
language:en
Short-container-title:Journal of Spectroscopy

Author:

Chikhirzhina Elena V.¹,Tatiana Starkova J.¹²,Polyanichko Alexander M.¹²

Affiliation:

1. Institute of Cytology of the Russian Academy of Sciences, Saint Petersburg 194064, Russia

2. Department of Molecular Biophysics, Faculty of Physics, Saint Petersburg State University, 1 Ulyanovskaya Street, Stary Petergoff, Saint Petersburg 198504, Russia

Abstract

Interaction of HMGB1 nonhistone chromosomal protein with DNA was studied using circular dichroism spectroscopy and thermal denaturation of DNA. Melting DNA in the complex was shown to be a biphasic process. The characteristic melting temperatures of unbound DNA and the DNA bound to HMGB1 in 0.25 mM EDTA solutions were found to beTmI=44.0±0.5°C andTmII=62.0±1°C, respectively. It was shown that the binding of the HMGB1 molecule affects the melting of the DNA region approximately 30 b.p. long.

Funder

Russian Foundation for Basic Researches

Publisher

Hindawi Limited

Subject

Spectroscopy,Atomic and Molecular Physics, and Optics,Analytical Chemistry

Link

http://downloads.hindawi.com/journals/jspec/2014/387138.pdf

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