Circadian Modulation of the Cl−Equilibrium Potential in the Rat Suprachiasmatic Nuclei

Author:

Alamilla Javier12ORCID,Perez-Burgos Azucena13,Quinto Daniel1,Aguilar-Roblero Raúl1

Affiliation:

1. División de Neurociencias, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Apartado Postal 70-253, 04510 México, DF, Mexico

2. Department of Psychology, Neuroscience & Behaviour, McMaster University, Hamilton, ON, Canada L8S 4K1

3. McMaster Brain-Body Institute, St Joseph’s Healthcare, Hamilton, ON, Canada L8N 4A6

Abstract

The suprachiasmatic nuclei (SCN) constitute a circadian clock in mammals, whereγ-amino-butyric acid (GABA) neurotransmission prevails and participates in different aspects of circadian regulation. Evidence suggests that GABA has an excitatory function in the SCN in addition to its typical inhibitory role. To examine this possibility further, we determined the equilibrium potential of GABAergic postsynaptic currents (EGABA) at different times of the day and in different regions of the SCN, using either perforated or whole cell patch clamp. Our results indicate that during the day most neurons in the dorsal SCN have anEGABAclose to −30 mV while in the ventral SCN they have anEGABAclose to −60 mV; this difference reverses during the night, in the dorsal SCN neurons have anEGABAof −60 mV and in the ventral SCN they have anEGABAof −30 mV. The depolarized equilibrium potential can be attributed to the activity of the Na(+)-K(+)-2Cl(−) (NKCC) cotransporter since the equilibrium potential becomes more negative following addition of the NKCC blocker bumetanide. Our results suggest an excitatory role for GABA in the SCN and further indicate both time (day versus night) and regional (dorsal versus ventral) modulation ofEGABAin the SCN.

Funder

Programa de Apoyo a Proyectos de Investigación e Innovación Tecnológica

Publisher

Hindawi Limited

Subject

General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

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